Exeter Chiefs forward Tom Johnson is one of 11 not required for England’s opening autumn international against Australia.
The 31-year-old was recalled to the senior squad following an injury to Leicester Tigers’ Tom Croft but has subsequently been released.
Lock Geoff Parling has been ruled out of Saturday’s game with concussion.
Parling was concussed during a contact session at the squad’s Pennyhill Park training base on Tuesday afternoon and will undergo the recommended graduated return to play protocol.
England head coach Stuart Lancaster said: “Geoff will be unavailable for Saturday’s game but will stay in camp with a view to returning to training next week.”
England squad to face Australia: Forwards: David Attwood (Bath Rugby), Dan Cole (Leicester Tigers), Dylan Hartley (Northampton Saints), Joe Launchbury (London Wasps), Courtney Lawes (Northampton Saints), Joe Marler (Harlequins), Ben Morgan (Gloucester Rugby), Chris Robshaw (Harlequins), Billy Vunipola (Saracens), Mako Vunipola (Saracens), David Wilson (Bath Rugby), Tom Wood (Northampton Saints), Tom Youngs (Leicester Tigers). Backs: Chris Ashton (Saracens), Mike Brown (Harlequins), Lee Dickson (Northampton Saints), Owen Farrell (Saracens), Toby Flood (Leicester Tigers), Ben Foden (Northampton Saints), Joel Tomkins (Saracens), Billy Twelvetrees (Gloucester Rugby), Marland Yarde (London Irish), Ben Youngs (Leicester Tigers).